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KMID : 1101320110430020075
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Korean Journal of Clinical Laboratory Science
2011 Volume.43 No. 2 p.75 ~ p.81
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The Effect of Chrysanthemum morifolium L. Extract on Cultured Neuroglial Cells Damaged by Glucose Oxidase
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Seo Young-Mi
Park Seung-Taeck
Rim Yo-Sup
Chung Ok-Bong
Jekal Seung-Joo
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Abstract
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To clarify the oxidative stress of reactive oxygen species (ROS) and the effect of Chrysanthemum morifolium L. (CM) flower extract on the cultured neuroglial cells (C6 glioma) damaged by ROS, cell adhesion effect was measured by colorimetric assay after cultured C6 glioma cells were treated with various concentrations of glucose oxidase (GO) for 5 hours. For the antioxidative effect of CM flower extract, cell adhesion activity (CAA), superoxide dismutase (SOD)-like activity and lactate dehydrogenase (LDH) activity were assessed against GO-induced cytotoxicity on same cultures. In this study, GO remarkably decreased CAA dose-dependently, and the XTT90 and XTT50 values were measured at 15 mU/mL and 50 mU/mL following the treatment of C6 glioma cells with 5∼60 mU/mL of GO. The CM flower extract significantly increased cell adhesion activity damaged by GO-induced cytotoxicity, and it also showed the SOD-like activity and the decrease of LDH activity. From these results, it is suggested that GO was cytotoxic on cultured C6 glioma cells, and CM flower extract showed antioxidative effects as shown by the increased CAA, SOD-like activity and the decrease of LDH activity on GO-induced cytotoxicity on the same cultures.
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KEYWORD
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Cell adhesion activity, Antioxidative effect, Oxidative stress
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